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Go to Editorial ManagerHigh-performance liquid chromatography (HPLC) is one of the most common analytical techniques used in pharmaceutical testing and in many other scientific fields. It is widely used because it can analyze different types of materials and give accurate and reliable results. HPLC is considered an important and trusted method in many laboratories. This narrative review gives a general explanation of HPLC, including its basic principles, simple instruments, and how the technique works with different samples. In pharmaceutical analysis, HPLC is mainly used in quality control laboratories. It is applied for drug assay, titration tests, impurity analysis, solubility studies, and stability testing. Many studies show that HPLC helps in ensuring the quality and safety of pharmaceutical products. In addition, HPLC is used in pharmacovigilance and toxicology to detect degradation products, identify counterfeit drugs, and find harmful or toxic compounds. These applications are important for protecting patient safety and supporting regulatory requirements. HPLC is also used in other fields such as environmental analysis, forensic science, and food analysis. In environmental studies, it helps detect pollutants and trace chemicals in water and soil samples. In forensic laboratories, HPLC is used to identify unknown substances in biological or chemical samples. In food analysis, it is applied to detect additives, contaminants, and residues. Although HPLC instruments have developed over time, proper method development, validation, and correct interpretation of results are still necessary to obtain reliable data for routine laboratory work and pharmaceutical regulatory use.
A reliable, rapid, and economical analytical method is required for the quality control of pharmaceutical preparations containing chlorphenamine maleate (CPM) and paracetamol (PCM). This study aimed to develop and validate a reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous determination and separation of these two common drugs. The chromatographic separation was performed on a C18 column (250×4.6 mm, 5 µm) using an isocratic mobile phase of phosphate buffer (pH 6), water, acetonitrile, and ethanol (70:15:15 v/v) at a flow rate of 0.8 ml/min. Detection was performed using a UV-Vis detector set at 228 nm. The method was validated for both pure substances and pharmaceutical preparations in accordance with standard guidelines, assessing linearity, accuracy, detection limit (LOD), and quantification limit (LOQ). The method successfully separated CPM and PCM with retention times of 3.2 min and 5.3 min, respectively, resulting in a total analysis time of less than 10 minutes. The method demonstrated excellent linearity over concentration ranges of 10-120 µg/mL for CPM (R² =1) and 10-110 µg/mL for PCM (R² = 0.9999). It showed high accuracy and reliability. The LOD was 0.06 µg/mL for CPM and 0.27 µg/mL for PCM, while the LOQ was 0.18 µg/mL for CPM and 0.82 µg/mL for PCM. The developed HPLC method is rapid, sensitive, precise, and economical. It is therefore suitable for routine quality control analysis and for the simultaneous quantification of chlorphenamine maleate and paracetamol in combined pharmaceutical dosage forms.
Moringa is a natural and cultivated variety of the genus Moringa of the family Moringaceae. The cultivation of Moringa is widespread due to multiple medicinal and healing properties. It is one of the richest plant sources of Vitamins A, B , C, D, E, K , in addition to proteins and minerals .The recent study aimed to phytochemically analyze Moringa oleifera grown in Iraqi environment and detection of some active compounds of the plant. Research reported that Moringa can support a healthy cardiovascular system, promote normal blood glucose levels, neutralize free radicals, provide excellent support of the body's anti inflammatory mechanisms, enrich anemic blood and support immune system. Moringa oleifera contains various phytoconstituents which contribute to its therapeutic uses such as alkaloids, saponins, tannins, steroids, phenolic acids, glycosides, flavonoids, and terpenoids. This study was able to identify the different phytochemical compounds present in Moringa cultivated in Iraq using preliminary chemical tests and to identify the presence of Quercetin using high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). The chromatographic techniques proved the presence of Quercetin by showing identical retention time and retention factor almost identical to that produced by quercetin standard.