Cover
Vol. 4 No. 1 (2026)

Published: June 1, 2026

Pages: 168-177

Research Article

Antioxidant and Anti-Angiogenesis Properties of Aristolochia Maurorum Using Ex Vivo, In Vitro, and In Vivo Studies

Abstract

Objective This study seeks to examine the anti-angiogenic characteristics of Aristolochia maurorum seed extract through various methodologies, including the extraction process, rat aortic ring assay, chorioallantoic membrane (CAM) assay, and DPPH assay for antioxidant assessment.

References

  1. Wang L, Liu WQ, Broussy S, Han B, Fang H. Recent advances of anti-angiogenic inhibitors targeting VEGF/VEGFR axis. Front Pharmacol [Internet]. 2024 Jan 4;14. Available from: https://www.frontiersin.org/articles/10.3389/fphar.2023.1307860/full
  2. Kopec M, Abramczyk H. The role of pro- and antiangiogenic factors in angiogenesis process by Raman spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc [Internet]. 2022 Mar;268:120667. Available from: https://www.sciencedirect.com/science/article/pii/S1386142521012440?via%3Dihub
  3. Teleanu RI, Chircov C, Grumezescu AM, Teleanu DM. Tumor angiogenesis and anti-angiogenic strategies for cancer treatment. J Clin Med [Internet]. 2019;9(1):84. Available from: https://www.mdpi.com/2077-0383/9/1/84
  4. Chung MS, Han SJ. Endometriosis-Associated Angiogenesis and Anti-angiogenic Therapy for Endometriosis. Front Glob Women’s Heal [Internet]. 2022 Apr 5;3. Available from: https://doi.org/10.3389/fgwh.2022.856316
  5. Qi S, Deng S, Lian Z, Yu K. Novel Drugs with High Efficacy against Tumor Angiogenesis. Int J Mol Sci [Internet]. 2022;23(13). Available from: https://www.mdpi.com/1422-0067/23/13/6934
  6. Najafi M. Tumor microenvironment : Interactions and therapy. 2019;(August 2018):5700–21. Availablefrom: https://pubmed.ncbi.nlm.nih.gov/30378106/
  7. Ricciuti B, Foglietta J, Bianconi V, Sahebkar A, Pirro M. Enzymes involved in tumor-driven angiogenesis: A valuable target for anticancer therapy. Semin Cancer Biol [Internet]. 2019;56:87–99. Available from: https://www.sciencedirect.com/science/article/pii/S1044579X17300433
  8. Margheri F, Anceschi C, Frediani E, Marzoppi A, Innocenti DD, Barletta E, et al. Posidonia oceanica Extract Inhibits VEGF-Induced Angiogenic and Oxidative Responses in Human Endothelial Colony- Forming Cells. 2025;15(5):1–42. Available from: https://www.mdpi.com/2039-4713/15/5/153
  9. İncilay TORUNOGLU E, Can AYTAR E, Durmaz A, Aydin B, Melda ÇOLAK A. Chemical Composition and Antioxidant Potential of Aristolochia maurorum Above-groud Extract: Phenolic and Phytochemical Analysis. ISPEC J Agric Sci [Internet]. 2024;8(3):737–46. Available from: https://ispecjournal.com/index.php/ispecjas/article/view/574
  10. Lerma-Herrera MA, Beiza-Granados L, Ochoa-Zarzosa A, López-Meza JE, Navarro-Santos P, Herrera-Bucio R, et al. Biological Activities of Organic Extracts of the Genus Aristolochia: A Review from 2005 to 2021. Molecules [Internet]. 2022 Jun;27(12). Available from: https://www.mdpi.com/1420-3049/27/12/3937
  11. Brown KJ, Maynes SF, Bezos A, Maguire DJ, Ford MD, Parish CR. A novel in vitro assay for human angiogenesis. Lab Invest [Internet]. 1996 Oct;75(4):539–55. Available from: https://pubmed.ncbi.nlm.nih.gov/8874385/
  12. Obaid KA. Anti-angiogenesis efficacy of the aqueous extract of Allium sativum and its combination with melatonin in an animal model: in vivo and ex vivo studies. PHARMACIA [Internet]. 2024;71:1–8. Available from: https://www.sciencedirect.com/science/article/pii/S0428029624000684
  13. Lyssy F, Brugger BA, Guettler J, Kupper N, Wernitznig S, Daxboeck C, et al. The chicken chorioallantoic membrane assay revisited – A face-lifted approach for new perspectives in placenta. 2025;166(June):77–84. Available from: https://doi.org/10.1016/j.placenta.2024.04.013
  14. BLOIS MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature [Internet]. 1958;181(4617):1199–200. Available from: https://www.nature.com/articles/1811199a0
  15. López-Bascón MA, Luque de Castro MD. Chapter 11 - Soxhlet Extraction. In: Poole CF, editor. Liquid-Phase Extraction [Internet]. Elsevier; 2020. p. 327–54. (Handbooks in Separation Science). Available from: https://www.sciencedirect.com/science/article/pii/B9780128169117000116
  16. TORUNOGLU Eİ, AYTAR EC, DURMAZ A, AYDIN B, ÇOLAK AM. Chemical Composition and Antioxidant Potential of Aristolochia maurorum Above-groud Extract: Phenolic and Phytochemical Analysis. ISPEC J Agric Sci [Internet]. 2024 Sep 1;8(3 SE-Articles):737–46. Available from: https://ispecjournal.com/index.php/ispecjas/article/view/574
  17. Kapoor A, Chen CG, Iozzo R V. A simplified aortic ring assay: A useful ex vivo method to assess biochemical and functional parameters of angiogenesis. Matrix Biol plus [Internet]. 2020 May;6–7:100025. Available from: https://www.sciencedirect.com/science/article/pii/S2590028520300065
  18. Nicosia RF. The aortic ring model of angiogenesis: a quarter century of search and discovery. J Cell Mol Med [Internet]. 2009 Oct;13(10):4113–36. Available from: https://pubmed.ncbi.nlm.nih.gov/19725916/
  19. Kennedy DC, Coen B, Wheatley AM, Mccullagh KJA. Microvascular Experimentation in the Chick Chorioallantoic Membrane as a Model for Screening Angiogenic Agents including from Gene- Modified Cells. 2022;(December 2021):1–76. Available from: https://www.mdpi.com/1422-0067/23/1/452
  20. Page PM. The Chorioallantoic Membrane Xenograft Assay as a Reliable Model for Investigating the Biology of Breast Cancer. 2026;(March 2023):1–64. Available from: https://www.mdpi.com/2072-6694/15/6/1704
  21. Singh S, Singh RP. In Vitro Methods of Assay of Antioxidants: An Overview. Food Rev Int [Internet]. 2008;24(4):392–415. Available from: https://doi.org/10.1080/87559120802304269
  22. Hassan NAA, Elkhouly AW, Ali MIK. Nutritional, antioxidant, and sensory properties of Ziziphus spina-christi fruit powder and its application in bakery products. Sci Rep [Internet]. 2025;15(1):43655. Available from: https://doi.org/10.1038/s41598-025-30233-9
  23. Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. 2022;27(4):1–49. Available from: https://www.mdpi.com/1420-3049/27/4/1326