Cover
Vol. 2 No. 2 (2024)

Published: August 31, 2024

Pages: 18-28

Original Article

The Effect of Methanolic extract of Eucalyptus sp. Leaves in Growth of some Opportunistic Fungi

Abstract

The recent study aimed to examine the Methanolic extract of Eucalyptus sp. leaves against some opportunistic fungi. The leaves of Eucalyptus sp. were collected, dried and then grinded. Powder of leaves was extracted using methanol and then filtered using filter papers. The filtrate was then centrifuged and then the filtrate was concentrated using a rotary evaporator. A stock solution was used to determine the inhibitory activity of the extract against opportunistic fungi. The results of detecting the active substances in the crude

References

  1. Bongomin F, Gago S, Oladele R, Denning D. Global and Multi-National Prevalence of Fungal Diseases—Estimate Precision. J. Fungi. 2017;3(4):57.
  2. Lu M, Li T, Wan J, Li X, Yuan L, Sun S. Antifungal effects of phytocompounds on Candida species alone and in combination with fluconazole. Int. J. Antimicrobial. Agents. 2017;49:125–136.
  3. Kannaiyan M, Meseret AG, Kanimozhi C, Thambidurai P, Ashokapuram Selvam S, Vinodhini R, et al. Prevalence of extended-spectrum beta-lactamase producing enterobacteriaceae members isolated from clinically suspected patients. Asian J. Pharma. Clin. Res. 2018;11:364.
  4. Roemer T, Krysan DJ. Antifungal Drug Development: Challenges, Unmet Clinical Needs, and New Approaches. Cold Spring Harbor Perspect. Med. 2014;4:019703.
  5. Mickymaray S, Al Aboody MS. In vitro antioxidant and bactericidal efficacy of 15 common spices: novel therapeutics for urinary tract infections? Medicina. 2019;55:289.
  6. Mickymaray S, Alturaiki W. Antifungal Efficacy of Marine Macroalgae against Fungal Isolates from Bronchial Asthmatic Cases. Molecules. 2018;23:3032.
  7. Moorthy K, Punitha T, Vinodhini R, Mickymaray S, Shonga A, Tomass Z, et al. Efficacy of different solvent extracts of Aristolochia krisagathra and Thottea ponmudiana for potential antimicrobial activity. J. Pharmacy Res. 2015;9:35–40.
  8. Thawabteh A, Juma S, Bader M, Karaman D, Scrano L, Bufo SA, et al. The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens. Toxins. 2019;11:656.
  9. Macphail M, Thornhill AH. "How old are the eucalypts? A review of the microfossil and phylogenetic evidence". Australian Journal of Botany. 2016;64(8):579.
  10. Dixit A, Rohilla A, Singh V. Eucalyptus globulus: A new perspective in therapeutics. Int J Pharm Chem Sci. 2012;1(4):1678–83.
  11. Boulekbache-Makhlouf L, Meudec E, Chibane M, et al. Analysis by high-performance liquid chromatography diode array detection mass spectrometry of phenolic compounds in fruit of Eucalyptus globulus cultivated in Algeria. J Agric Food Chem. 2010;58(24):12615–24.
  12. Emara S, Shalaby AE. Seasonal variation of fixed and volatile oil percentage of four Eucalyptus spp. related to lamina anatomy. Afr J Plant Sci. 2011;5(6):353–9.
  13. Okogun JI. Methods of Medicinal Plant Extract Preparation. National Institute for Pharmaceutical Research and Development (NIPRD); 2000.
  14. Kaur, Gupta S, Gautam P. Phytochemical analysis of Eucalyptus leaves extract. J Pharmacogn Phytochem. 2019;8(1):2442–2446.
  15. Forbes B, Sahm D, Weissfeld A. Bailey and Scott's: Diagnostic Microbiology. 12th ed. St. Louis, Mo.: Elsevier Mosby, USA; 2007. pp. 628–716.
  16. Balouiri M, Sadiki M, Ibnsouda S. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71–79.
  17. Halat D, Younes S, Mourad N, Rahal M. Allylamines, Benzylamines, and Fungal Cell Permeability: A Review of Mechanistic Effects and Usefulness against Fungal Pathogens. Membranes (Basel). 2022;12(12):1171.
  18. Kumar S, Pandey A. Chemistry and Biological Activities of Flavonoids: An Overview. Scientific World Journal. 2013;162750.
  19. Al Aboody MS, Mickymaray S. Anti-Fungal Efficacy and Mechanisms of Flavonoids. Antibiotics. 2020;9(2):45.
  20. Hasim S, Coleman J. Targeting the fungal cell wall: current therapies and implications for development of alternative antifungal agents. Future Med Chem. 2019;11(8):869–883.
  21. Zhou LJ, Li FR, Huang LJ, Yang ZR, Yuan S, Bai LH. Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern. Molecules. 2016;21(5):621.
  22. Lopez-Meneses AK, Plascencla-Jatomea M, Lizardi-Mendoza J, Rosas-Burgos EC, Luque-Alcaraz AG, Cortez-Rocha MO. Antifungal and antimycotoxigenic activity of essential oils from Eucalyptus globulus, Thymus capitatus and Schinus molle. Food Sci. Technol. 2015;35(4).
  23. Fradi A, Al-Araji A. Effect of Eucalyptus camaldulensis Terpens, Alkaloids and Phenols Against Fusarium oxysporum. Iraqi Journal of Science. 2015;56(4):2807–2810.
  24. Knobloch K, Pauli A, Iberl N, Weigand N, Weis HM. Antibacterial and antifungal properties of essential oil components. Journal of Essential Oil Research. 1989;1:119–128.
  25. Hiroshi N. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science. 1994;264:382–388.
  26. Simonetti G, Brasili E, Pasqua G. Antifungal Activity of Phenolic and Polyphenolic Compounds from Different Matrices of Vitis vinifera L. against Human Pathogens. Molecules. 2020;25(16):3748.