Abstract
Objective
Angiogenesis is the biological process that creates new capillary blood vessels from existing ones. This process is a big part of how tumors grow, spread, and spread to other parts of the body. The present study aimed to evaluate the anti-angiogenic and antioxidant activities of different extracts of Juglans regia L. green husk and to explore their potential therapeutic relevance.
Methods
The rat aortic ring angiogenesis experiment used albino male rats that were twelve to fourteen weeks old. The ethanolic extract of Juglans regia L. was prepared and solubilized in dimethyl sulfoxide (DMSO) to form a stock solution. The rat aortic ring assay was employed ex vivo to evaluate the anti-angiogenic characteristics of the plant extract.
Results
The aqueous extract showed the highest extraction yield (4.46%), followed by the ethanolic (2.15%) and chloroform extracts (1.9%). In the rat aortic ring assay, the ethanolic extract demonstrated the strongest anti-angiogenic activity (52.92% inhibition), followed by the aqueous (32.90%) and chloroform extracts (30.98%) compared with the negative control (p < 0.05). In the CAM assay, the ethanolic extract significantly reduced vascular density, producing approximately 65.4% inhibition of angiogenesis (p < 0.05). Furthermore, the ethanolic extract exhibited concentration-dependent antioxidant activity in the DPPH assay, with a maximum scavenging activity of 83.7% at 100 µg/mL and an IC50 value of approximately 23.3 µg/mL.
Conclusions
Juglans regia L. may represent a potential natural source of anti-angiogenic and antioxidant activity for the management of angiogenesis-related disorders.