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Go to Editorial ManagerObjective to find the potential anti–angiogenic and antioxidant effect of Anabasis setifera extracts. Methods The rat aortic ring anti-angiogenesis assay, DPPH radical scavenging assay, and chick chorioallantoic membrane (CAM) assay were carried out in the tissue culture laboratory of the Department of Pharmacology, College of pharmacy, Al-Nahrain University. Results The aqueous extract of Anabasis setifera demonstrated significant inhibition of microvessel outgrowth in the rat aortic ring assay by 71% , with 86.5% reduction of blood vessels growth In the CAM model, treatment resulted in a marked reduction in neovascularization compared with the control group. The extract also exhibited concentration-dependent antioxidant activity in the (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging assay Conclusions The water extract of Anabasis setifera . exhibits significant anti-angiogenic and antioxidant activities and may represent a promising natural source angiogenesis agents .
Objective: Angiogenesis is the formation of new blood vessels from pre-existing vasculature, a basic and tightly controlled biological process. Angiogenesis is essential for tissue regeneration, wound healing, and embryonic development under normal physiological conditions. However, under pathological conditions, dysregulated angiogenesis contributes to several diseases, including cancer, making it an important therapeutic target. Accordingly, natural products, including Capparis spinosa L. have attracted considerable attention as promising anti-angiogenic agents. C. spinosa is rich in bioactive phytochemicals such as flavonoids and phenolic compounds that have been reported to possess anti-angiogenic potential. This study aimed to evaluate the anti-angiogenic and antioxidant activities of Capparis spinosa leaf extracts using complementary ex vivo and in vivo models. Methods: The rat aortic ring anti-angiogenesis assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and chick chorioallantoic membrane (CAM) assay were carried out in the tissue culture laboratory of the Department of Pharmacology, College of Pharmacy, Al-Nahrain University. Results: The ethanolic extract demonstrated significant inhibition of microvessel outgrowth in the rat aortic ring assay, with an IC₅₀ value of 16.2 µg/mL and reduced neovascularisation in the CAM model, with an inhibition zone of 12±1.83 mm. The extract also exhibited concentration-dependent antioxidant activity in the DPPH assay. Conclusions: The ethanolic extract of Capparis spinosa L. exhibits significant anti-angiogenic and antioxidant activities and represents a promising natural source of compounds capable of modulating angiogenesis and oxidative stress, supporting its potential therapeutic applications.
Objective Angiogenesis is an essential process in tumor growth and progression, and thus it represents a promising therapeutic target. Lawsonia inermis (henna) is a widely-used traditional medicine with different biological applications, and its bioactive components, especially lawsone, showed anticancer activity. The objective of this research was to measure the anti-angiogenic and antioxidant properties of Lawsonia inermis leaf ethanolic extract in ex vivo and in vivo systems. Methods Soxhlet was used to prepare the ethanolic extract of the Lawsonia inermis leaf. The ex vivo rat aorta ring assay was used to test the anti-angiogenic activity at the concentrations of 100, 50, 25, 12.5, and 6.25 µg/mL. The in vivo chick chorioallantoic membrane (CAM) assay was employed to confirm the anti-angiogenic effect at a concentration of 10 mg/mL. DPPH radical scavenging assay was used to determine the antioxidant activity with a concentration range of 3.125 to 100 µg/mL. Results The ethanolic extract demonstrated high anti-angiogenic activity in the rat aorta ring with 65.82% inhibition at 100 µg/mL and dose-dependent inhibition with an IC 50 of 54.2 µg/mL. In the CAM assay, acetylsalicylic acid (positive control) resulted in complete suppression of vascularization, validating the assay system. The extract exhibited a concentration-dependent radical scavenging ability of DPPH radical with an IC 50 value of 0.05 µg/mL. Conclusions Lawsonia inermis ethanolic extract has strong anti-angiogenic and antioxidant properties, which implies its possible application as a treatment of angiogenesis-related disorders, such as cancer. The anti-angiogenic effect was confirmed in both ex vivo and in vivo models.
Objective Angiogenesis is the biological process that creates new capillary blood vessels from existing ones. This process is a big part of how tumors grow, spread, and spread to other parts of the body. The present study aimed to evaluate the anti-angiogenic and antioxidant activities of different extracts of Juglans regia L. green husk and to explore their potential therapeutic relevance. Methods The rat aortic ring angiogenesis experiment used albino male rats that were twelve to fourteen weeks old. The ethanolic extract of Juglans regia L. was prepared and solubilized in dimethyl sulfoxide (DMSO) to form a stock solution. The rat aortic ring assay was employed ex vivo to evaluate the anti-angiogenic characteristics of the plant extract. Results The aqueous extract showed the highest extraction yield (4.46%), followed by the ethanolic (2.15%) and chloroform extracts (1.9%). In the rat aortic ring assay, the ethanolic extract demonstrated the strongest anti-angiogenic activity (52.92% inhibition), followed by the aqueous (32.90%) and chloroform extracts (30.98%) compared with the negative control (p < 0.05). In the CAM assay, the ethanolic extract significantly reduced vascular density, producing approximately 65.4% inhibition of angiogenesis (p < 0.05). Furthermore, the ethanolic extract exhibited concentration-dependent antioxidant activity in the DPPH assay, with a maximum scavenging activity of 83.7% at 100 µg/mL and an IC50 value of approximately 23.3 µg/mL. Conclusions Juglans regia L. may represent a potential natural source of anti-angiogenic and antioxidant activity for the management of angiogenesis-related disorders.