The screening, production, and optimization of extracellular lipase from a fungus Aspergillus niger isolated from different sources were examined in this work. The isolated fungi were screened on tributyrin hydrolysis method to detect exogenous lipolytic activity. A promising lipase producing isolate of A. niger-6 was selected and identified as the highest lipaseproducing strain. The optimal conditions were determined for: inducer oil, carbon source, nitrogen source, initial pH value, incubation temperature, incubation period, inoculum size, and shaking speed for lipase production using one factor at a time approach. Maximum lipase production was observed in 1% olive oil, 2% glucose, 2g/l ammonium sulphate, pH6, 30°C, 5days, 1x106spores/ml-1, and 150 rpm, respectively.
The recent study aimed to examine the Methanolic extract of Eucalyptus sp. leaves against some opportunistic fungi. The leaves of Eucalyptus sp. were collected, dried and then grinded. Powder of leaves was extracted using methanol and then filtered using filter papers. The filtrate was then centrifuged and then the filtrate was concentrated using a rotary evaporator. A stock solution was used to determine the inhibitory activity of the extract against opportunistic fungi. The results of detecting the active substances in the crude